無(wú)需針對(duì)每種蛋白制備特異性抗體，無(wú)需構(gòu)建轉(zhuǎn)基因體系，高通量檢測(cè)轉(zhuǎn)錄因子及DNA結(jié)合蛋白的結(jié)合位點(diǎn)。
藍(lán)景科信為您提供DAP-seq全流程技術(shù)服務(wù)和個(gè)性化數(shù)據(jù)分析，具有100多個(gè)物種，2000多個(gè)轉(zhuǎn)錄因子的實(shí)驗(yàn)經(jīng)驗(yàn)，已協(xié)助很多客戶成功發(fā)表文章。例如：Molecular Plant，The Plant Cell，Plant Physiology，Plant Biotechnology Journal，Journal of Integrative Plant Biology，New Phytologist，Cell等。
在基因功能組學(xué)和表觀遺傳學(xué)研究中，轉(zhuǎn)錄因子結(jié)合位點(diǎn)（Transcription Factor Binding Sites, TFBS）的發(fā)掘一直是研究熱點(diǎn)之一。傳統(tǒng)的ChIP-seq（染色質(zhì)免疫共沉淀測(cè)序）方法，在抗體質(zhì)量很好的情況下能夠有效檢測(cè)到TFBS。然而，好的抗體可遇不可求，這限制了ChIP-seq更廣泛的應(yīng)用。
2016年，O'Malley RC等人在Cell上發(fā)表了使用DAP-seq技術(shù)，快速繪制轉(zhuǎn)錄因子調(diào)控靶向DNA區(qū)域圖譜的文章。2017年，Bartlett A等在Nature Protocols上發(fā)表了DAP-seq的實(shí)驗(yàn)方法。DAP-seq技術(shù)使TFBS的研究不再局限于任何生物，不再受抗體質(zhì)量的限制，為生命科學(xué)和醫(yī)學(xué)領(lǐng)域轉(zhuǎn)錄因子的研究提供了新型高效的工具。
技術(shù)流程
動(dòng)物：
驢、飛蝗、新孢子蟲、煙粉虱、草地貪夜蛾、斑點(diǎn)叉尾鮰、綿羊、褐飛虱、斜紋夜蛾、華貴櫛孔扇貝、曼氏血吸蟲
真菌：
擬輪枝鐮孢菌、豬苓真菌、意大利青霉、草酸青霉、金黃殼囊孢、靈芝、糙皮側(cè)耳、草菇、灰蓋鬼傘、蟲草、亞洲鐮刀菌、蝗綠僵菌、裂殖壺菌、禾谷鐮刀菌、元蘑、疫霉、高盧蜜環(huán)菌
細(xì)菌：
路德維希腸桿菌、嗜熱厭氧桿菌、生氮假單胞菌、布魯氏菌、肺炎克雷伯菌、類球紅細(xì)菌、紅桿菌科細(xì)菌、成團(tuán)泛菌、銅綠假單胞菌、伯克霍爾德菌
部分客戶發(fā)表文章：
Zhao H, Wan S, Huang Y, Li X, Jiao T, Zhang Z, Ma B, Zhu L, Ma F, Li M. The transcription factor MdBPC2 alters apple growth and promotes dwarfing by regulating auxin biosynthesis. Plant Cell. 2024 Feb 26;36(3):585-604. doi: 10.1093/plcell/koad297. (IF=11.6)
Yuan J, Liu X, Zhao H, Wang Y, Wei X, Wang P, Zhan J, Liu L, Li F, Ge X. GhRCD1 regulates cotton somatic embryogenesis by modulating the GhMYC3-GhMYB44-GhLBD18 transcriptional cascade. New Phytol. 2023 Oct;240(1):207-223. doi: 10.1111/nph.19120. (IF=9.4)
Yu Z, Chen X, Li Y, Shah SHA, Xiao D, Wang J, Hou X, Liu T, Li Y. ETHYLENE RESPONSE FACTOR 070 inhibits flowering in Pak-choi by indirectly impairing BcLEAFY expression. Plant Physiol. 2024 Jan 25:kiae021. doi: 10.1093/plphys/kiae021. (IF=7.4)
Cao X, Li X, Su Y, Zhang C, Wei C, Chen K, Grierson D, Zhang B. Transcription factor PpNAC1 and DNA demethylase PpDML1 synergistically regulate peach fruit ripening. Plant Physiol. 2023 Nov 22:kiad627. doi: 10.1093/plphys/kiad627. (IF=7.4)
Dong Q, Tian Y, Zhang X, Duan D, Zhang H, Yang K, Jia P, Luan H, Guo S, Qi G, Mao K, Ma F. Overexpression of the transcription factor MdWRKY115 improves drought and osmotic stress tolerance by directly binding to the MdRD22 promoter in apple. Hortic Plant J. 2023. doi: 10.1016/j.hpj.2023.05.005. (IF=5.7)
Fang Y, Wang D, Xiao L, Quan M, Qi W, Song F, Zhou J, Liu X, Qin S, Du Q, Liu Q, El-Kassaby YA, Zhang D. Allelic variation in transcription factor PtoWRKY68 contributes to drought tolerance in Populus. Plant Physiol. 2023. doi: 10.1093/plphys/kiad315. (IF=8.005)
Zhu J, Wei X, Yin C, Zhou H, Yan J, He W, Yan J, Li H. ZmEREB57 regulates OPDA synthesis and enhances salt stress tolerance through two distinct signalling pathways in Zea mays. Plant Cell Environ. 2023. doi: 10.1111/pce.14644. (IF=7.947)
Han P, Hua Z, Zhao Y, Huang L, Yuan Y. PuCRZ1, an C2H2 transcription factor from Polyporus umbellatus, positively regulates mycelium response to osmotic stress. Front Microbiol. 2023. 14:1131605. doi: 10.3389/fmicb.2023.1131605. (IF=6.064)
Zhang SL, Wang L, Yao J, Wu N, Ahmad B, Nocker S, Wu JY, Abudureheman R, Li Z, Wang XP. Control of ovule development in Vitis vinifera by VvMADS28 and interacting genes. Horticulture Research. 2023. doi: 10.1093/hr/uhad070. (IF=7.291)
Wang L, Tian T, Liang J, Li R, Xin X, Qi Y, Zhou Y, Fan Q, Ning G, Becana M, Duanmu D. A transcription factor of the NAC family regulates nitrate-induced legume nodule senescence. New Phytol. 2023. 238(5): 2113-2129. doi: 10.1111/nph.18896. (IF=10.323)
Sun Y, Han Y, Sheng K, Yang P, Cao Y, Li H, Zhu QH, Chen J, Zhu S, Zhao T. Single-cell transcriptomic analysis reveals the developmental trajectory and transcriptional regulatory networks of pigment glands in Gossypium bickii. Mol Plant. 2023. doi: 10.1016/j.molp.2023.02.005. (IF=21.949)
Liu Y, Liu Q, Li X, Zhang Z, Ai S, Liu C, Ma F, Li C. MdERF114 enhances the resistance of apple roots to Fusarium solani by regulating the transcription of MdPRX63. Plant Physiol. 2023. doi: 10.1093/plphys/kiad057. (IF=8.005)
Liu YN, Wu FY, Tian RY, Shi YX, Xu ZQ, Liu JY, Huang J, Xue FF, Liu BY, Liu GQ. The bHLH-zip transcription factor SREBP regulates triterpenoid and lipid metabolisms in the medicinal fungus Ganoderma lingzhi. Commun Biol. 2023. doi: 10.1038/s42003-022-04154-6. (IF=6.548)
Liu L, Chen G, Li S, Gu Y, Lu L, Qanmber G, Mendu V, Liu Z, Li F, Yang Z. A brassinosteroid transcriptional regulatory network participates in regulating fiber elongation in cotton. Plant Physiol. 2022. doi: 10.1093/plphys/kiac590. (IF=8.005)
Li M, Hou L, Zhang C, Yang W, Liu X, Zhao H, Pang X, Li Y. Genome-wide identification of direct targets of ZjVND7 reveals the putative roles of whole-genome duplication in Sour jujube in regulating xylem vessel differentiation and drought tolerance. Front Plant Sci. 2022. 13: 829765. doi: 10.3389/fpls.2022.829765. (IF=6.627)
Bi Y, Wang H, Yuan X, Yan Y, Li D, Song F. The NAC transcription factor ONAC083 negatively regulates rice immunity against Magnaporthe oryzae by directly activating transcription of the RING-H2 gene OsRFPH2-6. J Integr Plant Biol. 2022. doi: 10.1111/jipb.13399. (IF=9.106)
Guo X, Yu X, Xu Z, Zhao P, Zou L, Li W, Geng M, Zhang P, Peng M, Ruan M. CC-type glutaredoxin, MeGRXC3, associates with catalases and negatively regulates drought tolerance in cassava (Manihot esculenta Crantz). Plant Biotechnol J. 2022. doi: 10.1111/pbi.13920. (IF=13.263)
Chai Z, Fang J, Huang C, Huang R, Tan X, Chen B, Yao W, Zhang M. A novel transcription factor, ScAIL1, modulates plant defense responses by targeting DELLA and regulating gibberellin and jasmonic acid signaling in sugarcane. J Exp Bot. 2022. 73: 6727-6743. doi: 10.1093/jxb/erac339. (IF=7.298)
Li R, Zheng W, Yang R, Hu Q, Ma L, Zhang H. OsSGT1 promotes melatonin-ameliorated seed tolerance to chromium stress by affecting the OsABI5-OsAPX1 transcriptional module in rice. Plant J. 2022. 112: 151-171. doi: 10.1111/tpj.15937. (IF=5.726)
Li Q, Zhou L, Chen Y, Xiao N, Zhang D, Zhang M, Wang W, Zhang C, Zhang A, Li H, Chen J, Gao Y. Phytochrome interacting factor regulates stomatal aperture by coordinating red light and abscisic acid. Plant Cell. 2022. 34: 4293-4312. doi: 10.1093/plcell/koac244. (IF=12.085)
Luo M, Lu B, Shi Y, Zhao Y, Wei Z, Zhang C, Wang Y, Liu H, Shi Y, Yang J, Song W, Lu X, Fan Y, Xu L, Wang R, Zhao J. A newly characterized allele of ZmR1 increases anthocyanin content in whole maize plant and the regulation mechanism of different ZmR1 alleles. Theor Appl Genet. 2022. 135: 3039-3055. doi: 10.1007/s00122-022-04166-0. (IF=5.574)
Wei H, Xu H, Su C, Wang X, Wang L. Rice CIRCADIAN CLOCK ASSOCIATED 1 transcriptionally regulates ABA signaling to confer multiple abiotic stress tolerance. Plant Physiol. 2022. 190: 1057-1073. doi: 10.1093/plphys/kiac196. (IF=8.005)
Tang N, Cao Z, Yang C, Ran D, Wu P, Gao H, He N, Liu G, Chen Z. A R2R3-MYB transcriptional activator LmMYB15 regulates chlorogenic acid biosynthesis and phenylpropanoid metabolism in Lonicera macranthoides. Plant Sci. 2021. 308: 110924. doi: 10.1016/j.plantsci.2021.110924. (IF=5.363)
Liang S, Gao X, Wang Y, Zhang H, Yin K, Chen S, Zhang M, Zhao R. Phytochrome-interacting factors regulate seedling growth through ABA signaling. Biochem Biophys Res Commun. 2020. 526: 1100-1105. doi: 10.1016/j.bbrc.2020.04.011. (IF=3.322)
Yao J, Shen Z, Zhang Y, Wu X, Wang J, Sa G, Zhang Y, Zhang H, Deng C, Liu J, Hou S, Zhang Y, Zhang Y, Zhao N, Deng S, Lin S, Zhao R, Chen S. Populus euphratica WRKY1 binds the promoter of H+-ATPase gene to enhance gene expression and salt tolerance. J Exp Bot. 2020. 71: 1527-1539. doi: 10.1093/jxb/erz493.(IF=5.36)
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